In neurodegenerative diseases, age-related decline in intracellular protein degradation is considered to cause accumulation and aggregation of misfolded protein in neurons, leading to neurodegeneration. The autophagy-lysosome system consists of three pathways: macroautophagy, microautophagy and chaperone-mediated autophagy (CMA). There are two major protein degradation systems: the ubiquitin-proteasome system (UPS) and the autophagy-lysosome system, which primarily degrade short-lived and long-lived proteins, respectively. In neurons, removal of misfolded proteins by protein degradation systems is important for various neuronal functions and survival. The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Sports and Culture and by grants from the Takeda Science Foundation, the Uehara Memorial Foundation, the Naito Foundation, Suzuken Memorial Foundation, the Tokyo Biochemical Research Foundation and the Japanese Smoking Research Association. Received: Accepted: JanuPublished: February 7, 2012Ĭopyright: © 2012 Seki et al. Chu, University of Pittsburgh, United States of America (2012) Establishment of a Novel Fluorescence-Based Method to Evaluate Chaperone-Mediated Autophagy in a Single Neuron. Citation: Seki T, Yoshino K-i, Tanaka S, Dohi E, Onji T, Yamamoto K, et al.

Chaperone mediated autophagy